Coding

Part:BBa_K2705001:Design

Designed by: Danqing Tong   Group: iGEM18_NKU_CHINA   (2018-09-30)


LacI (reverse)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The negative feedback system of lactose operon was applied to seive out cells with specific feature.


Source

The part was isolated from vector pHT01 by PCR.

References